High‐Throughput Drug Screening: A Microfabricated Sandwiching Assay for Nanoliter and High‐Throughput Biomarker Screening (Small 15/2019)
نویسندگان
چکیده
منابع مشابه
FISSR-PCR: a simple and sensitive assay for highthroughput genotyping and genetic mapping.
The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and auto...
متن کاملMicrofabricated Physiological Models for In Vitro Drug Screening Applications
Microfluidics and microfabrication have recently been established as promising tools for developing a new generation of in vitro cell culture microdevices. The reduced amounts of reagents employed within cell culture microdevices make them particularly appealing to drug screening processes. In addition, latest advancements in recreating physiologically relevant cell culture conditions within mi...
متن کاملAssay for Drug Screening in Rheumatoid Arthritis
Fleming researchers have developed an actin-based cellular assay for drug discovery in treating Rheumatoid Arthritis (RA). The assay uses primary synovial fibroblasts (SF) isolated from the joints of proprietary arthritic transgenic mice overexpressing human TNF. The assay accurately quantifies the intracellular level of F-actin using fluorescencently-labelled phalloidin. Reduced F-actin levels...
متن کاملNew Platforms For Drug Screening And Toxicology: Necessity Or Need?
The liver is the largest internal organ in the human body that is responsible for more than 500 vital functions, including biosynthesis of major plasma proteins, immunity against infectious pathogens, balancing energy metabolism and xenobiotics biotransformation (1). One of the main functions of the liver is an important role in drug metabolism (2). Since developing new drug compounds into mark...
متن کاملAttomolar Sensitive Functional Proteomic Assay for Biomarker Detection and Drug Screening
Kinases are known to play important roles in cellular signaling, thus exhibiting tremendous potential as effective biomarkers. Here, we developed a multiplexed microfluidic assay capable of optically detecting kinase activity at concentrations as low as 10 attoMolar (7 active molecules=12x10 moles) with a dynamic range of five orders of magnitude within one hour.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Small
سال: 2019
ISSN: 1613-6810,1613-6829
DOI: 10.1002/smll.201970078